The gym
Brian's going at it pretty hard... and Ted and Em take the opportunity to rub in the age difference between him and Justin, which is terrible timing and hitting right at the heart of Brian's insecurities. This ep focuses a lot on Justin, but I think a big part of it is about Brian being the one insecure in their relationship....Justin had 206 to feel insecure after Brian fucked zucchini guy, and now it's Brian's turn to feel it after Justin kissed the virgin. I think the biggest problem in their relationship, the one that leads to the break up, is that they BOTH have all these insecurities where their relationship is concerned... which are luckily mostly erased after their reunion in 308. But for now, Brian still feels that a break up is inevitable, and the way he acts because of this feeling—never wanting to commit too much of himself—is what ultimately makes Justin feel unwanted and makes the breakup inevitable.
Anyway, Brian turns it around and points out that Em is dating a 103 year old, lol. Em says that when they said goodnight, they also said goodbye.