Loki had managed to forget the whole 'surprise daughter' part of the night and focused his attention on Darcy. He didn't say anything at first, but it wasn't hard to realize he was upset.
He was blue, for starters.
"And your mother is not in this universe?" The question was asked calmly, but he was anything but calm.
Funny how he could have issues with something like that. He really wasn't the best villain in the world.