In the usual tradition of 1610, this is a summary of Betty in that world: "Betty Ross is a cunning and shameless public relations mastermind with an obsessive work ethic, an almost invariably heartless attitude, and a knack for deceit and manipulation."
Sam would absolutely know who she is. But then Bruce is pretty fucked up there too and he's largely gotten used to MCU Bruce so he'll give her the benefit of the doubt. (You know, not to mention... everyone else.)
I don't think my 616 ones know that version of her.