"Yes, sir," Fitz noted to the order of not discussing the matter. He gave the slightest huff that Loki's moving had caused the machine to have to re-scan. It beeped quietly, then reset at his temple again and started turning. Fitz looked to Agent Coulson, then back to Simmons, frowning. The pixies continues their slow hovering, one posing in front of a gunman's nose, cause him to tilt his head back oh-so-slightly before it hovered to a new position, moving in neat array with the others.
As Jemma was talking on the phone, he moved subtly to the machines, adjusting some settings on the centrifuge. He met her gaze, giving a small nod. Calling Thor a diplomatic liaison worked to stave off questions for now. In the meantime, there was going to be a following of Coulson's orders and no one else's.
Loki relaxed a little, focusing on her, green eyes searching at hers while she spoke. He finally gave a smile, only seen by the squint of his eyes, but his brow was still pinched in the middle. Still his hand turned up, cooperating, and he closed his eyes and pulled in deeper breaths through his nose. The small body relaxed some, thinking to himself how Thor was his brother, and he was of Asgard. It was a small thing, a needle, wasn't it? Even so his breath stopped for a brief second on the pinch and slide. Then he opened his eyes and watched the rest of the draw, jaw flexing around the gag as the green eyes flicked back and forth.
Agent Fitz then stepped to Coulson's side. "While the DNA is running, you'll have to collaborate the machine further if you want to question him. That means asking him pre-um-preliminary questions. I--you're probably better at knowing what needs asked for that." Fitz was not an interrogator. He could only take the readings and tweak the machine for what brain and vital signatures Coulson needed.